Cell-Cycle-Dependent Pharmacology of Methotrexate in HL-60
نویسندگان
چکیده
منابع مشابه
evaluation of effect of as2o3 on cell growth, cell cycle and apoptosis in human leukemia cell line hl-60
introduction : arsenic trioxide (as2o3) causes antitumor effects by various mechanisms, including degradation of pml-rara in the acute promyelocytic cells, growth inhibition and induction of apoptosis. however, the precise mechanisms of these processes remain incompletely understood. the purpose of this study was to evaluate the effects of arsenic trioxide on cell growth, cell cycle regulation ...
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Using rhodamine 123 to stain mitochondria of the human cell line HL-60, we have followed their increase over the cell cycle by flow cytometry. A near-linear synthesis of mitochondrial mass was shown to occur over the cell cycle. A comparison with the cell's DNA synthesis pattern obtained by the same technique established a common time-base. The mitochondrial synthesis curve changes with culture...
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Recent research suggests the vivid possibility of using nitrite therapy against various pathological conditions. Moreover, chronic nitrite therapy offers protection against ischemia and augments endothelial cell proliferation through unknown mechanisms. Nitrite-mediated augmentation in the number of circulating neutrophils has also been reported; however, the exact mechanism is not known. In th...
متن کاملDifferences in cell cycle kinetics during induced granulocytic versus monocytic maturation of HL-60 leukemia cells.
Leukemia cells (HL-60) were induced to mature towards granulocytic and monocytic phenotypes using 1.1% dimethyl sulfoxide and 5 X 10(-7) M 1-beta-D-arabinofuranosylcytosine, respectively. Granulocytic maturation was accompanied by a slight decrease in cell volume and in total cell protein, but with an increase in acid phosphatase. DNA histograms showed that after 7 days there was a decrease in ...
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Expression of the proliferation-associated nucleolar antigen pi 20 was studied by flow cytometry in human quiescent and phytohemagglutininstimulated lymphocytes, as well as in human lymphocytic (MOLT-4) and promyelocytic (HL-60) cell lines. Divariate analysis of pl20 and DNA content made it possible to correlate p120 expression with cell position in the cycle. Proliferating lymphocytes and MOLT...
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ژورنال
عنوان ژورنال: Journal of Pharmacological Sciences
سال: 2005
ISSN: 1347-8613,1347-8648
DOI: 10.1254/jphs.fp0050761